Fig. 3

Lj-1-60 suppresses the proliferation of melanoma cells in vitro. a Melanoma cells Sk-Mel-5 and Sk-Mel-28 seeded into 96-well plates were treated with various concentrations of Lj-1-60 for 0, 24, 48, 72 h, cell viability was tested by CCK-8 kit, the value of IC50 was calculated by Graphpad prism 6. All sample represented at least for three replicates with three independent experiments, ****P < 0.0001 by two-way ANOVA. b Cell viability in non-tumor cells including PIG1 and H9C2 was measured as described in “Methods”. Data are Mean ± SD (n = 4 wells), ****P < 0.0001 by two-way ANOVA. c Inhibition of Fyn and treated with 2 μM Lj-1-60 for 48 h in melanoma cells. Cell viability was examined by CCK-8 assay, ***P < 0.001, ****P < 0.0001. d Melanoma cells were seeded into 6-well plate with complete medium overnight, cells were replaced with medium containing 2 μM Lj-1-60 for 24 h, and complete medium cultured for another 2 weeks, the number of clones was stained with crystal violet and counted with software image J. The data represented here are expressed as the mean(n = 3) ± S.D. **P < 0.01