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Fig. 4 | Cancer Cell International

Fig. 4

From: A novel chalcone derivative suppresses melanoma cell growth through targeting Fyn/Stat3 pathway

Fig. 4

Lj-1-60 induces melanoma cell apoptosis and cell cycle arrest in G2/M phase. a Cell cycle distribution was detected by flow cytometry treated with 2 μM Lj-1-60 for 48 h in melanoma cells Sk-Mel-5 and Sk-Mel-28, and DMSO as vehicle according to the manufacture’s instruction. The results represented the mean (n = 3) ± SD of each group, *P < 0.05. b Melanoma cells Sk-Mel-5 and Sk-Mel-28 treated with 2 μM Lj-1-60 for 48 h, and the percentage of apoptosis was examined by flow cytometry as described in the "Methods" with Annexin V and PI double staining. Data were represented as mean (n = 3) ± SD, *P < 0.05, ***P < 0.001. c Melanoma cells Sk-Mel-5 and Sk-Mel-28 treated with 2 μM Lj-1-60 for 48 h, Total cell lysates were quantified and subjected to western blotting detected with the indicated antibodies

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