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Fig. 2 | Cancer Cell International

Fig. 2

From: Exploiting collateral sensitivity controls growth of mixed culture of sensitive and resistant cells and decreases selection for resistant cells in a cell line model

Fig. 2

Proliferation of mono- and coculture of sensitive and resistant cells under ribociclib treatment. a Representative images of time course spheroid growth of spheroids of different compositions. Images present the merged images of the Venus and mCherry channels. White bars correspond to 1000 µm. b Time course growth of spheroids presented on a. Data points show average spheroid areas of three replicates, error bars show standard deviation if it is larger than the size of the data point. Vertical lines represent statistical comparisons of the spheroid area between untreated sensitive vs. mixed (red), untreated sensitive and untreated resistant (brown), treated sensitive and mixed (purple) and treated sensitive and resistant (orange) spheroids on Day 21. Asterisks mark statistical significance (p < 0.05). Further statistical analysis including all time points and raw data are presented in Additional file 1: Table S1. c Representative FACS analysis of mixed spheroids on Day 21 on untreated (left) and ribociclib treated (right) spheroids initiated in a composition of 50% sensitive and 50% resistant cells. Samples are identical to the mixed samples on a (second and fifth rows). d Proportion of sensitive cells in untreated and ribociclib treated cocultured spheroids based on FACS analysis. Bars show the average of three replicates, error bars show standard deviation. Asterisk marks statistical significance (p < 0.05)

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