Fig. 3

Comparing the transcriptional program of CAMA-1 and CAMA-1_ribociclib_resistant cells reveals collateral sensitivity to Wee-1 inhibition. a Schematic representation of significantly altered Hallmark pathways between untreated CAMA-1 and CAMA-1_ribociclib_resistant cell lines. Positive normalized enrichment scores (NES) corresponds to enriched pathways in CAMA-1 (blue circles), while pathways with negative NES values (red circles) are enriched in CAMA-1_ribociclib_resistant cells. Additional enrichment scores for Hallmark pathways can be found in Additional file 1: Table S5. b Heatmap of all genes included in the Hallmark G2/M pathway in untreated CAMA-1 and CAMA-1_ribociclib_resistant cells. Representative genes of the pathway are labeled. Heatmap with all genes labeled can be found in Additional file 3: Figure S2. c Dose–response curves of CAMA-1 and CAMA-1_ribociclib_resistant cells under different concentrations of Wee-1 inhibitor adavosertib treatment. Cells were treated with increasing concentration of adavosertib for 96 h, after which viability was measured using CellTiterGlo Chemiluminescent kit. The measured luminescence was normalized to the average of the lowest applied concentration (0.01 nM). Data points show the average of three replicates, error bars show standard deviation if it is larger than the size of the data point