Fig. 2
From: MIAT inhibits proliferation of cervical cancer cells through regulating miR-150-5p
Ectopic expression of MIAT inhibited malignant progression in cervical cancer cells. a HeLa and HT-3 cells were transfected with either empty vector (E.V) or MIAT plasmid using lipofectamine 2000. The expression efficiency was measured by real-time PCR at 24 h post-transfection. ****p < 0.0001; b Cell viability was determined in MIAT-proficient HeLa and HT-3 cells with CCK-8 kit. n.s: no significance, *** p < 0.001, ****p < 0.0001; c Cell proliferation was measured by 1-week clonogenic assay. The representative image was acquired under optical microscope with crystal violet staining. d The anchorage-independent growth of both MIAT-proficient and naïve HeLa and HT-3 cells was evaluated with soft agar assay (left pane), the corresponding statistical results from three individual fields were shown in the right pane, ****p < 0.0001