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Fig. 1 | Cancer Cell International

Fig. 1

From: Antitumor efficacy of cytosine deaminase-armed vaccinia virus plus 5-fluorocytosine in colorectal cancers

Fig. 1

Characterization of vaccinia VG9-CD. a Schematic illustration of the shuttle plasmid pCB-CD. b Western blot proved the expression of CD in vaccinia VG9-CD recombinants. 0 represents vaccinia VG9-TK− as a negative control. M represents protein Marker. Bottom graph showed relative CD levels that were normalized to ACTB. c CD activity determined by the conversion rate of 5-FC to 5-FU. Vaccinia lysates obtained from 10 vaccinia VG9-CD recombinants were added to 5-FC solution. The reaction run for 3 h at 37 °C, then the concentration of 5-FC and 5-FU were detected by the Waters Breeze HPLC unit. The conversion rate of 5-FC to 5-FU was calculated by comparing the proportion of peak area of 5-FU in the total peak areas of 5-FC and 5-FU

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Cancer Cell International

ISSN: 1475-2867

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