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Fig. 1 | Cancer Cell International

Fig. 1

From: RETRACTED ARTICLE: Circ-DENND4C up-regulates TCF4 expression to modulate hepatocellular carcinoma cell proliferation and apoptosis via activating Wnt/β-catenin signal pathway

Fig. 1

Circ-DENND4C regulated HCC cell growth, invasion and stemness. a qRT-PCR examined circ-DENND4C expression in HCC cells and normal liver epithelial cells. b, c Gel electrophoresis and ActD treatment verified circular characteristic of circ-DENND4C. d qRT-PCR verified knockdown efficiency of sh-circ-DENND4C in HCC cells. e, f. Immunofluorescence staining assay (scale bar = 30 μm) and colony formation assay detected the proliferation of HCC cells transfected with sh-circ-DENND4C#1/2. g Cell cycle was analyzed by flow cytometry upon circ-DENND4C knockdown. h TUNEL assay (scale bar = 100 μm) revealed HCC cell apoptosis with transfection of sh-circ-DENND4C#1/2. i The protein levels of Cyclin D1, CDK4, Bcl-2 and Bax were determined through western blot analysis. j Transwell assay (scale bar = 100 μm) detected cell invasion in HCC cells transfected with sh-circ-DENND4C#1/2. k, l qRT-PCR and western blot detected mRNA and protein levels of stemness biomarkers in HCC cells upon circ-DENND4C depletion. *P < 0.05, **P < 0.01

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