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Fig. 3 | Cancer Cell International

Fig. 3

From: RETRACTED ARTICLE: Circ-DENND4C up-regulates TCF4 expression to modulate hepatocellular carcinoma cell proliferation and apoptosis via activating Wnt/β-catenin signal pathway

Fig. 3

Circ-DENND4C sponged miR-195-5p. a, b Subcellular fractionation assay and FISH assay (scale bar = 10 μm) were carried out to detect the subcellular location of circ-DENND4C in HCC cells. c RNA pull down assay detected enrichments of 9 candidate miRNAs in no-biotin/biotin-labeled circ-DENND4C probe. d RIP assay detected miR-195-5p, circ-DENND4C and miR-6838-5p enrichments in Ago2 and IgG group. e qRT-PCR examined miR-195-5p and miR-6838-5p expressions in HCC cells and normal THLE-3 cells. f Binding sequences between circ-DENND4C and miR-195-5p was predicted from starBase. g qRT-PCR verified the overexpression efficiency of miR-195-5p. h Luciferase activity of circ-DENND4C-wt/mut reporters was examined after miR-195-5p overexpression. i RNA pull down assay examined enrichment of circ-DENND4C pulled down by biotinylated miR-195-5p-wt/mut. **P < 0.01, n.s. meat no significance

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Cancer Cell International

ISSN: 1475-2867

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