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Fig. 5 | Cancer Cell International

Fig. 5

From: RETRACTED ARTICLE: Circ-DENND4C up-regulates TCF4 expression to modulate hepatocellular carcinoma cell proliferation and apoptosis via activating Wnt/β-catenin signal pathway

Fig. 5

Circ-DENND4C mediated HCC cells proliferation, apoptosis and invasion via up-regulating TCF4. a, b Immunofluorescence staining assay (scale bar = 30 μm) and colony formation assay detected the proliferation of HCC cells transfected with sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+pcDNA3.1-TCF4. c Flow cytometry was used to analyze cell cycle in the groups of sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+pcDNA3.1-TCF4. d TUNEL assay (scale bar = 100 μm) revealed HCC cell apoptosis by transfecting sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+pcDNA3.1-TCF4. e Cyclin D1, CDK4, Bcl-2 and Bax protein levels were tested in each group. f Transwell assay (scale bar = 100 μm) detected cell invasion in HCC cells transfected with sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+pcDNA3.1-TCF4. g, h qRT-PCR and western blot detected the protein levels of stemness biomarkers in HCC cells transfected with sh-NC, sh-circ-DENND4C#1 and sh-circ-DENND4C#1+pcDNA3.1-TCF4. *P < 0.05, **P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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