Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: Untargeted LC–MS/MS analysis reveals metabolomics feature of osteosarcoma stem cell response to methotrexate

Fig. 2

Measurement of the expression of targeted genes, ALDH1, Sox2, CD133, OCT-4 and CD 177 in OS and their SCs. a Immunoblotting assay of ALDH1, Sox-2 and GAPDH, b Statistical analysis of the expression of ALDH1 and Sox-2, c Immunofluorescence assay of CD133 and OCT-4 (Original magnification: 200 ×), d Statistical analysis of the expression of CD133 and Oct-4, e Statistical analysis of the expression of CD117 at transcriptional level by qRT-PCR. *compared with their parental osteosarcoma cells, P < 0.05. SC stem cell, OS osteosarcoma, ALDH1 aldehyde dehydrogenase 1, Sox-2 sex determining region Y (SRY)-like box 2, OCT-4 octamer‑binding transcription factor 4, FITC fluorescein isothiocyanate, DAPI 4′,6-diamidino-2-phenylindole, GAPDH glyceraldehyde-3-phosphate dehydrogenase

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us