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Fig. 3 | Cancer Cell International

Fig. 3

From: Untargeted LC–MS/MS analysis reveals metabolomics feature of osteosarcoma stem cell response to methotrexate

Fig. 3

The measurement of tumorigenic potential in osteosarcoma (OS) and their stem cells. a Proliferation rate of OS and their stem cells was determined by CCK8 assay; b Migration and invasion rate was determined by Matrigel® assay; c Orthotopic xenograft models were performed and at 3 weeks after injection, the tumor volume was measured; d, e Methotrexate cytotoxicity assays were performed by using CCK8 assay and IC50 was estimated with GraphPad Prism for Windows. *compared with their parental OS cells, P < 0.05. SC stem cell

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Cancer Cell International

ISSN: 1475-2867

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