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Fig. 3 | Cancer Cell International

Fig. 3

From: Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells

Fig. 3

Palbociclib decreases glucose carbon incorporation into PPP intermediates, in part, via decreased G6PD activity. a Fractional enrichment of m + 5 labeled nucleotides and nucleosides, and b m + 7 sedoheptulose 7-phosphate following 48-h treatment with 1 μM palbociclib. Values represent mean ± SD, analyzed by unpaired student’s t-test (n = 6, technical replicates). Statistical significances between each group are as follows: **p < 0.01 and ***p < 0.001. c G6PD activity assessed in A549 cells following knockdown of RB and 48-h treatment with 1 μM palbociclib. dg qPCR analysis of glucose 6-phosphate dehydrogenase (G6PD), phosphoribosyl pyrophosphate synthetase 2 (PRPS2), and ribonucleotide reductase 1/2 (RRM1/2) in A549 cells following knockdown of RB and 48-h treatment with 1 μM palbociclib. cg Values represent mean ± SD, analyzed by two-way ANOVA with Sidak’s post hoc multiple comparisons (n = 3, independent experiments). Statistical significances between each group are as follows: **p < 0.01

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