Fig. 5
From: Palbociclib treatment alters nucleotide biosynthesis and glutamine dependency in A549 cells
Palbociclib increases PC-mediated anaplerosis in A549 cells. a Cartoon of [U-13C]-glucose fate mapping through the 1st turn of the TCA cycle. Red circles are 13C labeling indicative of pyruvate dehydrogenase activity, green circles are 13C labeling indicative of pyruvate carboxylase activity, and white circles are unlabeled 12C. b–e Fractional enrichment of the TCA metabolites b citrate, c fumarate, d malate, and e aspartate in A549 cells following 48-h treatment with 1 μM palbociclib. Values represent mean ± SD, analyzed by unpaired student’s t-test for each metabolite isotopologue (vehicle vs. palbociclib) (n = 6, technical replicates). Statistical significances between each group are as follows: ***p < 0.001