Fig. 3

MiR-802 is a target of circDONSON in GC cells. a Sequence alignment of miR-802 with the putative binding sites in circDONSON. b Dual-luciferase reporter assay in AGS/DDP and HGC-27/DDP cells co-transfected with the reporter plasmid (or the corresponding mutant reporter) and miR-802 mimic or miR-NC mimic. c qRT-PCR analysis of miR-802 in AGS/DDP and HGC-27/DDP cells transfected with si-NC or si-circDONSON. d, e qRT-PCR analysis of miR-802 expression in normal gastric mucosa from noncancerous patients and gastric cancer tissues (N = 60), as well as in DDP responsive (N = 35) and non-responsive GC tissues (N = 25). f qRT-PCR analysis of miR-802 expression in normal GES-1 cells, GC cell lines (AGS and HGC-27), and DDP-resistant GC cell lines (AGS/DDP and HGC-27/DDP). g Correlation analysis between miR-802 and circDONSON expression in GC tissues. n = 3, *P < 0.05