Fig. 6
MiR-802 alleviates DDP resistance in GC cells by targeting BMI1. a qRT-PCR analysis of miR-802 in AGS/DDP and HGC-27/DDP cells transfected with miR-802 mimic or miR-NC mimic. b, c Levels detection of the mRNA and protein of BMI1 in AGS/DDP and HGC-27/DDP cells transfected with pcDNA-BMI1 or pcDNA-NC. AGS/DDP and HGC-27/DDP cells were transfected with miR-802 mimic, miR-NC mimic, miR-802 mimic + pcDNA-NC, or miR-802 mimic + pcDNA-BMI1. d CCK-8 assay of the viability and IC50 value of AGS/DDP and HGC-27/DDP cells after exposure to a series dose of DDP (0.125, 0.25, 0.5, 1, 2, 4, or 8 µM). e Colony formation assay of the colony-forming ability of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment. f Flow cytometry of the apoptosis of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment. g Western blot analysis of Cyclin D1 and p27 levels in AGS/DDP and HGC-27/DDP cells. n = 3, *P < 0.05