Fig. 3

CircOSBPL10 upregulated UBE2Q1 expression by competitively binding with miR-1179 in CC. a Venn diagram showed the overlaps of potential miR-1179 targets predicted by PITA, microT, PicTar, miRmap. b The binding capacity between miR-1179 and four mRNAs was verified through RNA pull-down assay. c, d The expression of UBE2Q1 in SiHa and HeLa cells transfected with different plasmids was detected via RT-qPCR and western blot. e UBE2Q1 expression in CC cell lines and H8 cells was detected via RT-qPCR. f The binding sites between UBE2Q1 and miR-1179 obtained from starBase were displayed. g The interaction between UBE2Q1 and miR-1179 was validated by luciferase reporter assay. h RIP analysis revealed that circOSBPL10, miR-1179 and UBE2Q1 co-existed in RISCs (RNA-induced silencing complexes). i RT-qPCR and western blot were utilized to detect the expression of UBE2Q1 in indicated cells. **P < 0.01