Fig. 5

FOXA1 activated circOSBPL10 expression in CC. a The efficacy of FOXA1 knockdown and overexpression in SiHa and HeLa cells was obtained through RT-qPCR analysis. b The expression of circOSBPL10 in different groups was detected by RT-qPCR. c The DNA motif of FOXA1 was obtained from JASPAR database. d Full-length of OSBPL10 promoter was fragmented into 5 pieces (P1-5), and JASPAR predicted a potential binding site for FOXA1 to OSBPL10 promoter at P4 region. e ChIP assay proved that the P4 region of OSBPL10 promoter was recognized by FOXA1 in both SiHa and HeLa cells. f The interaction between OSBPL10 promoter and FOXA1 at above predicted sites was testified by luciferase reporter assay. **P < 0.01