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Fig. 2 | Cancer Cell International

Fig. 2

From: Immune-related long non-coding RNA signature identified prognosis and immunotherapeutic efficiency in bladder cancer (BLCA)

Fig. 2

Elevated expression of 5 selected lncRNAs in paracancerous tissues and normal urothelium cell compared with BLCA tissues and cells. ae qRT-PCR analysis indicates the transcriptional level of AC005014.2 (a), AC010503.4 (b), AL450384.2 (c), LINC00930 (d) and SH3BP5-AS1 (e) in distinct malignancy BLCA cells (EJ, UM-UC-3, T24, 5637, J82, SCaBER and RT-4) and normal immortalized urothelium cell SV-HUC-1. The GAPDH allele is used as a loading control. The gene expression of SV-HUC-1 cells was characterized as the control to compare the relative gene expression in each cell, *p < 0.05, **p < 0.01, ***p < 0.001, ns: p > 0.05. f–j) qRT-PCR analysis exhibits the expression of AC005014.2 (f), AC010503.4 (g), AL450384.2 (h), LINC00930 (i) and SH3BP5-AS1 (j) at the transcription level in BLCA tissues compared with that in paired paracancerous tissues. The GAPDH allele is used as an internal control. The difference between BLCA and paired pancancerous tissues were measured with Student t tests. The p value was indicated in the figures

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