Fig. 3

MiR-15a-5p was a target of PVT1, and miR-15a-5p inhibition reversed the effects of PVT1 interference in PCa cells. a, b The expression of miR-15a-5p was measured in PCa tissues and cell lines by qRT-PCR. c The correlation between miR-15a-5p expression and PVT1 expression was analyzed according to Spearman’s correlation coefficient. d The binding sites between PVT1 and miR-15a-5p were predicted by starBase v2.0, and their relationship was verified by dual-luciferase reporter assay. e The expression of miR-15a-5p was affected by the change of PVT1 expression. f The expression of miR-15a-5p, g cell proliferation, h apoptosis, i migration (×100), j invasion (×100) and k the levels of Vimentin, E-cad, Caspase-3 and CyclinD1 were determined in PCa cells transfected with si-PVT1, si-NC, si-PVT1 + miR-15a-5p inhibitor and si-PVT1 + inhibitor NC, respectively. *P < 0.05, **P < 0.01, ***P < 0.001