Fig. 2
From: β-catenin promotes MTX resistance of leukemia cells by down-regulating FPGS expression via NF-κB
β-catenin regulated the protein expression of FPGS and NF-κB p65. a Western blot analysis of FPGS and p65 expression after β-catenin knockdown in Jurkat cells. b Western blot analysis of FPGS and p65 expression after β-catenin knockdown in MOLT4 cells. c Western blot analysis of FPGS and β-catenin expression after treatment with 10−8 M MTX for 2 weeks in MOLT4 cells. d The mRNA level of β-catenin in sh-Control or sh-β-catenin by qPCR in Jurkat cells. e The mRNA level of β-catenin in sh-Control or sh-β-catenin by qPCR in MOLT4 cells. f The mRNA level of FPGS in sh-Control or sh-β-catenin by qPCR in Jurkat cells. g The mRNA level of FPGS in sh-Control or sh-β-catenin by qPCR in MOLT4 cells. h Jurkat cells transfected with control shRNA or β-catenin-targeting shRNA were treated with CHX. The immunoblotting of FPGS and β-catenin (left) and the relative levels of FPGS (right) were quantifified