Fig. 3
From: β-catenin promotes MTX resistance of leukemia cells by down-regulating FPGS expression via NF-κB
NF-κB p65 regulated FPGS transcription. a FPGS mRNA expression in Jurkat cells treated with DMSO or NF-κB inhibitor PDTC by qPCR. Columns, mean of 3 individual experiments; Bars, SD;*, p < 0.05, **, p < 0.01, ***, p < 0.001. b FPGS mRNA expression in Jurkat cells treated with H2O or NF-κB stimulant LPS by qPCR. Columns, mean of 3 individual experiments; Bars, SD;*, p < 0.05, **, p < 0.01, ***, p < 0.001. c Schematic diagram of NF-κB p65 binding sites in about 700 bp of the human FPGS promoter and the construction of MUT-1 and MUT-2. d Luciferase reporter assay in HEK-293 cells transfected with pGL3-Basic, pGL3-FPGS-WT, pGL3-FPGS-MUT-1, or pGL3-FPGS-MUT-1 plasmid. e, f ChIP and Re-ChIP assays in Jurkat cells