Fig. 2

OIP5-AS1 promotes PDAC cell proliferation and migration. a The level of OIP5-AS1 was measured in PANC-1 cells transfected with sh-OIP5-AS1-1, sh-OIP5-AS1-2 or CFPAC-1 cells transfected with pcDNA3.1 empty vector or pcDNA3.1/OIP5-AS1. Results were evaluated by RT-qPCR (**P < 0.01). The transfected cells were used for subsequent experiments. b, c CCK-8 and colony formation assays detected measured the cell proliferation ability in indicated groups. (**P < 0.01). d Transwell assay detected cell migration ability in PANC-1 cells transfected with indicated plasmids (**P < 0.01). e Western blot researched the effects of OIP5-AS1 depletion or up-regulation on proteins involved in EMT process and ERK signaling pathway in cells under different conditions (**P < 0.01). *P< 0.05, and **P < 0.01 indicated data were statistically significant