Fig. 3

OIP5-AS1 acts as miR-429 sponge and is inversely correlated with miR-429 in PDAC. a StarBase conjectured the binding sites between OIP5-AS1 and miR-429. b Luciferase activity of indicated reporters under diverse transfections was examined by luciferase reporter assay (**P < 0.01). c RIP assay was applied to detect the enrichment of OIP5-AS1 and miR-429 in anti-IgG- or anti-Ago2-induced immunoprecipitates (**P < 0.01). d The harvest of above RNAs precipitated in RIP assays were processed with agarose gel electrophoresis after semi-quantitative PCR. e RT-qPCR tested the level of OIP5-AS1 and miR-429 in indicated PDAC cells (**P < 0.01). f The expression of miR-429 in 110 pairs of PDAC tissues was analyzed by RT-qPCR (**P < 0.01). g The correlation between miR-429 and OIP5-AS1 expression in PDAC tissues was tested by Pearson’s correlation analysis (P < 0.001). **P < 0.01 indicated data were statistically significant