Fig. 2

LINC00242 knockdown represses GC cell migration, invasion, and in vitro angiogenesis. a The expression of LINC00242 in HGC27 cells upon LINC00242 knockdown was determined by RT-qPCR. b HGC27 cell viability was measured by CCK-8 assay. c Representative view of HGC27 cells with LINC00242 knockdown invading from Matrigel-coated chambers into lower ones and statistics of invading cells. d Representative view of HGC27 cells with LINC00242 knockdown migrating from upper transwell chambers into lower ones and statistics of migrating cells. e Representative Western blots of N-cadherin, Vimentin, MMP-2, MMP-9 and their quantitation analyses in HGC27 cells upon LINC00242 knockdown. fIn vitro tube formation of HBMVECs in HGC27 cells upon LINC00242 knockdown. Results are presented as mean ± S.D. of three technical replicates. *indicates p < 0.05 compared with si-NC by unpaired t-test (panel a, c–f) or repeated measurements ANOVA with Bonferroni corrections (panel b)