Fig. 3
From: Activation of the LINC00242/miR-141/FOXC1 axis underpins the development of gastric cancer
LINC00242 interacts with miR-141 and positively regulats FOXC1, a target gene of miR-141. a The predicted localization of LINC00242. The abscissa represents different cell lines and the ordinate represents the localization of LINC00242; the histogram above the 0 line represents the expression mainly in the cytoplasm, and the histogram below the 0 line represents the expression mainly in the nucleus. b A heat map illustrating differentially expressed miRNAs from GC-related GSE26595 dataset. c The intersecting miRNAs from the GSE26595 dataset and the RAID database (http://www.rna-society.org/raid/search.html). d A heat map illustrating some significantly upregulated genes from the GSE13861 dataset. e The intersecting genes from the mirDIP (http://ophid.utoronto.ca/mirDIP/index.jsp#r), TargetScan (http://www.targetscan.org/vert_71/) and DIANA (http://diana.imis.athena-innovation.gr/DianaTools/index.php?r=microT_CDS/index) databases and the GSE13861 dataset. f Subcellular localization of LINC00242 detected by FISH assay (× 400, scale bar = 25 μm). Green represents LINC00242 expression and blue represents the stained nucleus. g The binding of LINC00242 and FOXC1 to AGO2 assessed by RIP assay. * indicates p < 0.05 compared with IgG. h Putative miR-141 binding sites in the FOXC1 3′UTR by a web-available prediction (www.targetscan.org). i The luciferase activity at the promoter of the reporter gene containing FOXC1-WT and FOXC1-MUT upon miR-141 mimic transfection. * indicates p < 0.05 compared with mimic NC. j Expression of FOXC1 binding to using anti-AGO2 antibody in upon LINC00242 knockdown detected by RIP assay. * indicates p < 0.05 compared with si-NC. k Expression of miR-141 and FOXC1 in cells upon si-LINC00242 transfection detected by RT-qPCR, normalized to U6 and GAPDH respectively. * indicates p < 0.05 compared with si-NC. l Expression of FOXC1 in cells transfected with miR-141 mimic detected by RT-qPCR, normalized to GAPDH. * indicates p < 0.05 compared with mimic NC. m Expression of FOXC1 in cells transfected with si-LINC00242 or miR-141 inhibitor detected by RT-qPCR, normalized to GAPDH. Results are presented as mean ± S.D. of three technical replicates. * (compared with si-NC + inhibitor NC) and # (compared with si-LINC00242 + inhibitor NC) indicate p < 0.05 by unpaired t-test or one-way ANOVA followed by Tukey’s test