Fig. 4

NORAD negatively regulated miR-495-3p expression in PCa cells. a The binding sites between NORAD and miR-495-3p were predicted with starBase v2.0 database. b, c The luciferase activities of luciferase reporter vectors of NORAD MUT and NORAD WT in 22Rv1 and LNCaP cells transfected with miR-495-3p or miR-NC were determined with dual-luciferase reporter assay. d, e RIP assay was performed to verify the relationship between miR-495-3p and NORAD. f RNA pull-down assay was employed to determine whether NORAD can be pull-down by miR-495-3p in both 22Rv1 and LNCaP cells. g Pearson’s correlation analysis method was employed to assess the correlation between NORAD and miR-495-3p in PCa tissues. h The expression of miR-495-3p in both 22Rv1 and LNCaP cells transfected with si-NORAD, si-NC, pcDNA-NORAD or pcDNA was detected by qRT-PCR. The experiments were performed in triplicate. ***P < 0.001