Fig. 6

MiR-495-3p targeted TRIP13 in PCa cells. a The binding sites of miR-495-3p in the 3′-UTR of TRIP13 were predicted with starBase v2.0 database. b, c Dual-luciferase reporter assay was carried out to detect the luciferase activity of luciferase reporter vector of TRIP13 3′-UTR-MUT or TRIP13 3′-UTR-MUT in 22Rv1 and LNCaP cells transfected with and miR-495-3p or miR-NC. d RNA pull-down assay was executed to verify the relationship between miR-495-3p and TRIP13. e, f QRT-PCR or western blot analysis was conducted to assess the expression of TRIP13 mRNA and protein in PCa tissues and adjoining healthy tissues. g, h The expression levels of TRIP13 mRNA and protein in 22Rv1, LNCaP, and RWPE-1 cells were detected by qRT-PCR or western blot analysis. i Pearson’s correlation analysis method was used to analyze the correlation between TRIP13 and miR-495-3p in PCa tissues. j The expression of TRIP13 protein in 22Rv1 and LNCaP cells transfected with anti-miR-495-3p, anti-miR-NC, miR-NC, or miR-495-3p was detected by western blot analysis. The experiments were performed in triplicate. ***P < 0.001