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Fig. 1 | Cancer Cell International

Fig. 1

From: Cytotoxicity study of the interleukin-12-expressing recombinant Newcastle disease virus strain, rAF-IL12, towards CT26 colon cancer cells in vitro and in vivo

Fig. 1

Viral copy number of AF2240-i and rAF-IL12 detected in CT26 cells at 24, 48, and 72 h post-infection as measured by RT-qPCR analysis based on NDV Fusion (F) gene for viral replication kinetics. The copy number was calculated based on the formula generated from the qPCR standard curve of the NDV: X = (y−58.149)/−3.371; where X is the viral copy number; y is the value mean Cq; 58.149 is the y-intercept value; and −3.371 is the slope of the standard curve. Data are presented as mean ± S.E.M from triplicate determinations. Statistically significant differences between the means were determined by One-Way ANOVA followed by Duncan post hoc test. Differences were considered significant when the *p ≤ 0.05

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