Fig. 6

a Tumour sections assayed by DeadEnd colorimetric TUNEL system to indicate cell apoptosis in CT26 tumour-bearing Balb/c mice: a positive control (sample treated with DNAse I); b negative control; c tumour treated with AF2240-i; d tumour treated with rAF-IL12. Brown stained nuclei in the black circle indicate DNA fragmentation and nuclear condensation. Magnification: 100x; b The number of apoptotic cells per tumour section from the four aforementioned groups after 28 days of treatment. A total number of 200 cells per tumor section was used for the quantification analysis in the TUNEL assay. Data are presented as mean ± S.E.M. of six mice per group. Mean values with statistical difference at p ≤ 0.05 between groups are indicated with *