Fig. 2

YY1 transcriptionally activates LINC00667 in CRC cells. a All potential transcription factors in the upstream of LINC00667 were subjected to qRT-PCR analysis in CRC cells with the normal colon epithelial cells as the negative control. Results were shown as a heatmap. b, c The mRNA and protein levels of five candidate transcription factors were detected in cells transfected with specific shRNAs. Results were examined through qRT-PCR and western blot after 48 h’ transfection. N = 3 in each group. d Luciferase reporter assays were performed to measure the luciferase activity of reporter vector containing the whole sequence of LINC00667 promoter after co-transfected with specific shRNAs for 48 h. N = 3 in each group. e The levels of YY1 mRNA and protein in CRC cells and normal FHC cell were measured by qRT-PCR and western blot, respectively. N = 3 in each group. f LINC00667 expression was tested via qRT-PCR after silencing of YY1. N = 3 in each group. g DNA motif of YY1 transcription factor downloaded from JASPAR was shown. h Three binding sequences of YY1 in LINC00667 promoter were exhibited. The role of YY1 as a transcription factor of LINC00667 was verified by ChIP assay in HCT116 and LoVo cells. N = 3 in each group. ^*P < 0.05, ^**P < 0.01 compared to control group