Fig. 4

NFAT5 is a target gene of miR-411-3p in OSCC. a 28 mRNAs which had the binding site with miR-411-3p were predicted by starBase. b The qRT-PCR analysis was utilized to screen out the mRNAs which could be inhibited by NFAT5 depletion and miR-411-3p overexpression. c The expressions of TLL2, MGAT4A, RAB21 and NFAT5 in SCC-4 and SCC-9 cells through qRT-PCR. d The binding site of NFAT5 and miR-411-3p. e RIP assay was adopted to test the relationship between TTN-AS1, miR-411-3p and NFAT5. f The interference efficiency of miR-411-3p was tested by qRT-PCR analysis. g The expression of NFAT5 was detected when NFAT5 and miR-411-3p was silenced. h The interference efficiency of NFAT5 was tested by qRT-PCR analysis. i, j Cell proliferation was evaluated through colony formation and EdU experiments when NFAT5 was knocked down. k, l Cell apoptosis was measured through flow cytometry and TUNEL experiments after inhibiting NFAT5. m Wound healing assays were carried out for estimating cell migration after NFAT5 was subjected to inhibition. **P < 0.01