Fig. 1

The expression of Tim-3 and galectin-9 in cervical cancer tissues and the genes methylation level. a, b An overview of mRNA levels of HAVCR2 and LGALS9 in cervical cancer based on Oncomine database. c The correction between HAVCR2 and LGALS9 in cervical cancer, analyzed by GEPIA database. d, e The protein levels of Tim-3 and galectin-9 in cervical cancer (Ca) (n = 24), para-carcinoma (Cap) (n = 24) and normal cervical tissues (NC) (n = 16) detected by western blotting. Blot images of four representative samples are shown from each group. f Predicted CpG islands in the promoter regions of HAVCR2 and LGALS9. Numbers indicate the positions in bp relative to the transcription start site. The blue region represents the CpG islands and the red vertical bars are the CpG loci in these input sequences. g, h Methylation level of HAVCR2 and LGALS9 promoter regions in cervical cancer (Ca) (n = 9) and NC (n = 9) detected by MS-PCR. (M methylated, U unmethylated); methylated and unmethylated levels of genes were quantified as M/M + U% and U/M + U%, respectively. i Positive immunohistochemistry staining of Tim-3 and galectin-9 and negative control in representative cervical cancer samples. Magnification, ×400. **P < 0.01, ns not significant