Fig. 6
From: SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer
SUV39H1 mediated Tim-3 and galectin-9 expression through DNA methylation in vivo. a SiHa-SUV39H1 and HeLa-SUV39H1 tumor xenografts in nude mice. b Tumors formed from SiHa-SUV39H1 and HeLa-SUV39H1 cells as well as tumor growth curves. c The tumors weight formed from SiHa-SUV39H1 and HeLa-SUV39H1. d–f Western blotting results of SUV39H1, H3K9me3, DNMT3A, Tim-3 and galectin-9 in SiHa-SUV39H1 and HeLa-SUV39H1 cells formed tumors. g The methylation level of HAVCR2 and LGALS9 promoter regions were monitored by MS-PCR in tumor tissues. h, i Chromatin was cross‑linked, fragmented and immunoprecipitated with either IgG (mock) or anti‑H3K9me3 ChIP‑grade antibody and the purified DNA was used to amplify with respective primer pairs for the indicated four regions in the DNMT3A promoter in qPCR. The enrichment of H3K9me3 on DNMT3A promoter relative to IgG in tumor tissues. *P < 0.05, **P < 0.01