SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

Zhang, Li; Tian, Sijuan; Zhao, Minyi; Yang, Ting; Quan, Shimin; Yang, Qing; Song, Lihua; Yang, Xiaofeng

doi:10.1186/s12935-020-01380-y

Cancer Cell International

Table 1 Primer sequences

From: SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancer

Name	Application	Sequence
DNMT3A-ChIP-F1	ChIP-qPCR	ATCATCAGTAGGGCGGGGTGGCCAC
DNMT3A-ChIP-R1	ChIP-qPCR	CTCCAATGCTTCCAGGTCCCTCCGT
DNMT3A-ChIP-F2	ChIP-qPCR	TTGGAGAACCTCCCGAAGGAAAACC
DNMT3A-ChIP-R2	ChIP-qPCR	GCCACCCTTTTAGCGTCACAGAACC
DNMT3A-ChIP-F3	ChIP-qPCR	CGTTGGGGGGGCGGGTGCTGGGCTG
DNMT3A-ChIP-R3	ChIP-qPCR	TGACTGGCACAGGACATGGCGTGCT
DNMT3A-ChIP-F4	ChIP-qPCR	CATGGGGAAGGAGAACAGCCCCCAC
DNMT3A-ChIP-R4	ChIP-qPCR	GCACTGGAAGACTGAAAGATTTCAT
HAVCR2-ChIP-F1	ChIP-qPCR	GTGGAAAAAATCTGTCACTTAGGGG
HAVCR2-ChIP-R1	ChIP-qPCR	ATTTTTAGTAGAGACGGGGTTTCTC
HAVCR2-ChIP-F2	ChIP-qPCR	CCTGTAATCCCAGCTACTCAGGAGG
HAVCR2-ChIP-R2	ChIP-qPCR	CTTGTTCAATGTGTGTACTTCCCAT
HAVCR2-ChIP-F3	ChIP-qPCR	CCCAATGCATTTAATGGCATAAATG
HAVCR2-ChIP-R3	ChIP-qPCR	CAGCCACACTCCCATAACTGAGGTA
HAVCR2-ChIP-F4	ChIP-qPCR	GGAACTCAACACTTTCTGATCATTC
HAVCR2-ChIP-R4	ChIP-qPCR	GACTTTGACCTTCAAACTTCCAACT
LGALS9-ChIP-F1	ChIP-qPCR	GGTAGAGTAAAATGTACAGATCCTG
LGALS9-ChIP-R1	ChIP-qPCR	GCGAGACCTTGTCTCTACTAAAAAT
LGALS9-ChIP-F2	ChIP-qPCR	TCAGCCTCCCAATGTGCTGAATTAC
LGALS9-ChIP-R2	ChIP-qPCR	CCAGATCCAAACTTGACTTGAAGTG
LGALS9-ChIP-F3	ChIP-qPCR	TCCTGTGGCCTAGCTCCTTTTTATT
LGALS9-ChIP-R3	ChIP-qPCR	AGAAAAACTGCTTGGTGAGTTGTAA
LGALS9-ChIP-F4	ChIP-qPCR	CACATATGTTTTCCTTTCTCTTGGG
LGALS9-ChIP-R4	ChIP-qPCR	ACACCTGTGGTCTCAGCTACATGGG
HAVCR2-ML	MS-PCR	TATAAAATGAGAAATTGGTCGGGCG
HAVCR2-MR	MS-PCR	TTACAAACATATACCACCACCCCGA
HAVCR2-UL	MS-PCR	GAAATTGGTTGGGTGTGGTGGTTAT
HAVCR2-UR	MS-PCR	TATACCACCACCCCAAATAATTTTA
LGALS9-9-ML	MS-PCR	TTTTCGAGATAGGTTTGCGATTTTG
LGALS9-9-MR	MS-PCR	AATACCGACACCCTTCAATCACCAC
LGALS9-9-UL	MS-PCR	GAGTTTTTGAGATAGGTTTGTGATT
LGALS9-9-UR	MS-PCR	ATACCAACACCCTTCAATCACCACA
SUV39H1-sense	Gene silencing	CCUUCGUGUACAUCAAUGATT
SUV39H1-anti-sense	Gene silencing	UCAUUGAUGUACACGAAGGTT
DNMT3A-sense	Gene silencing	GCCAAGGUCAUUGCAGGAATT
DNMT3A-anti-sense	Gene silencing	UUCCUGCAAUGACCUUGGCTT
Negative control-sense	Gene silencing	UUCUCCGAACGUGUCACGUTT
Negative control-sense	Gene silencing	ACGUGACACGUUCGGAGAATT

ChIP chromatin immunoprecipitation, RT-PCR reverse transcription-polymerase chain reaction, F forward primer, R backward primer, MS-PCR methylation-specific-polymerase chain reaction, ML/UL methylation/unmethylation forward primer, MR/UR methylation/unmethylation backward primer

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ISSN: 1475-2867

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