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Fig. 3 | Cancer Cell International

Fig. 3

From: A newly identified lncRNA H1FX-AS1 targets DACT1 to inhibit cervical cancer via sponging miR-324-3p

Fig. 3

H1FX-AS1 acted as a sponge for miR-324-3p. a Cellular sublocalization of the H1FX-AS1 in the SiHa and HeLa cells were determined by the nuclear and cytoplasmic separation experiments, U6 and GAPDH are used as internal references for nuclear and cytoplasmic expression, respectively. The potential binding sites of H1FX-AS1 with miR-324-3p were identified: the schematic representation of the potential binding sites of H1FX-AS1 with miR-324-3p predicted by lncBASE (b), which was verified by double luciferase reporter assay in the SiHa (left panel) and HeLa (right panel) cells after co-transfection of H1FX-AS1 WT or mutated reporter with or without miR-324-3p mimics (c); miR-324-3p in the cell lysates of SiHa (left panel) and HeLa (right panel) cells was further pulled down and enriched with biotin labeled H1FX-AS1 specific probe (d); the miR-324-3p expression level was confirmed to be negatively related with the H1FX-AS1 expression level after checking the miR-324-3p expression level in the SiHa (left panel) and HeLa (right panel) cells with (OE-H1FX-AS1) or without (vector) H1FX-AS1 over-expression (e). **p < 0.01

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