Fig. 3

Interaction of miR-138-5p and FOXC1. a Bioinformatics analysis websites (miRDB, TargetScan and StarBase) showed the potential downstream target gene of miR-138-5p; b qRT-PCR was used to detect the differential expression of the potential downstream target gene of miR-138-5p in NC mimic and miR-138-5p mimic, respectively; c Western Blotting verified the expression level of FOXC1 after transfection of NC mimic and miR-138-5p mimic PCa cell lines, respectively; d qRT-PCR verified the expression level of FOXC1 after transfection of NC mimic and miR-138-5p mimic PCa cell lines, respectively; e qRT-PCR verified the expression level of miR-138-5p after transfection of pcDNA3.1-NC and pcDNA3.1-FOXC1 in PC-3 and DU-145 cell lines, respectively; f, g Dual luciferase reporter assays demonstrated direct targeting of miR-138-5p to FOXC1; h A significant negative correlation between miR-138-5p and FOXC1 expression in PCa tissues. Data are mean ± SD, *p < 0.05, **p < 0.01