Fig. 2

hsa_circ_0000069 promotes proliferation, migration and invasion in CC cell lines. a qRT-PCR analysis of hsa_circ_0000069 expression in SiHa and HeLa cells transfected with NC or si-hsa_circ_0000069 (si-1# and 2#). b, c Cell counting kit-8 and colony formation assays were used to measure the proliferation ability of siHa and HeLa cells transfected with NC or si-hsa_circ_0000069 (si-1# and 2#). d, e Transwell assays were used to determine the effects of hsa_circ_0000069 on CC cell migration and invasion. All data is representative of three independent experiments and expressed as mean ± SD. P value in Fig. 2b was determined by a one-way ANOVA, and the others were determined by two-tailed Student’s unpaired t-test, **P < 0.01. NC: negative control; si: small interfering. f Representative western blots of the protein expression levels for cell proliferation and migration biomarkers in siHa and HeLa cells transfected with NC or si-hsa_circ_0000069 (si-1# and 2#). GAPDH was used as an internal reference