Fig. 4

hsa_circ_0000069 directly interacts with miR-873-5p. a The mRNA levels of nuclear control (U6), cytoplasmic control (GAPDH) and hsa_circ_0000069 were analyzed using qRT‐PCR in nuclear and cytoplasmic fractions. b A predicted binding site of miR-873-5p within hsa_circ_0000069 by bioinformatic analysis using the CircInteractome database (https://circinteractome.nia.nih.gov/). And the binding sequences “AGUUCCUGA” in hsa_circ_0000069 were mutated to “CAGGAAGUC” for generating Mut‐hsa_circ_0000069. c, d Luciferase reporter and RNA pull-down assays were used to determine the interaction between hsa_circ_0000069 and miR-873-5p. e Relative mRNA expression of miR-873-5p in hsa_circ_0000069-knockdown SiHa and Hela cells. f Relative mRNA expression of miR-873-5p in CC tissues and adjacent normal tissues was examined using qRT‐PCR. g Spearman correlation analysis between miR-873-5p and hsa_circ_0000069 expressions in 50 pairs of CC tissues. All data are representative of three independent experiments and shown as mean ± SD. P values were determined by two-tailed Student’s unpaired t-test, **P < 0.01; ns, not significant