Fig. 6

hsa_circ_0000069 promotes TUSC3 expression by sponging miR-873-5p. a A predicted binding site of miR-873-5p within the TUSC3 3′-UTR region using Targetscan. And the binding sequences “GUUCCUG” in hsa_circ_0000069 were mutated to “UCCAAGU” for generating Mut-TUSC3. Luciferase reporter assays were used to evaluate the interaction between TUSC3 3′-UTR and miR-873-5p. b Relative protein levels of TUSC3 in miR-873-5p-overexpressed SiHa and HeLa cells were measured using densitometric quantification by image J. Representative western blots of SiHa and Hela cells transiently transfected with miR-NC or miR-873-5p mimics. The data are normalized to GAPDH and presented as mean ± SD. of two experiments. c Relative protein expression levels of TUSC3 in hsa_circ_0000069-knockdown or miR-873-5p inhibitor-treated SiHa and HeLa cells were measured using densitometric quantification by image J. Representative western blots of SiHa and Hela cells transiently transfected with NC or si-hsa_circ_0000069 (si-1#) or both si-1# and miR-873-5p inhibitor. The data are normalized to GAPDH and presented as mean ± SD. of two experiments. d Spearman correlation analysis between miR-873-5p and TUSC3 expressions in CC tissues. Data are shown as mean ± SD. P values were determined by two-tailed Student’s unpaired t-test, **P < 0.01. inh: inhibitor