Fig. 7

Restoration of TUSC3 reversed the effects of hsa_circ_0000069 knockdown in CC cells. a Relative protein expression levels of TUSC3 in hsa_circ_0000069-knockdown, or both hsa_circ_0000069-knockdown and TUSC3-overexpressed SiHa and HeLa cells were measured using densitometric quantification by image J. Representative western blots of SiHa and Hela cells transiently transfected with NC or si-hsa_circ_0000069 (si-1#), or both si-1# and pcDNA3.1-TUSC3. The data are normalized to GAPDH and presented as mean ± SD. of two experiments. b, c Cell counting kit‐8 and colony formation assays were used to measure the proliferation of SiHa and HeLa cells. d, e Transwell assays were used to determine the migration and invasion of SiHa and HeLa cells. Data are representative of three independent experiments and shown as mean ± SD. P value in Fig. 7b was determined by a one-way ANOVA, and the others were determined by two-tailed Student’s unpaired t-test, **P < 0.01. NC negative control, si small interfering, OE Overexpression