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Table 1 Enrichment/isolation approaches of CTCs based on the inherent characteristics

From: Potential theranostics of circulating tumor cells and tumor-derived exosomes application in colorectal cancer

Total approach Methods/kits
Biological (Immuno-affinity)
 Negative selection Rosettesep [50]/Easysep [51]/Magnetic-activated cell sorting (MACS) [52]/Fluorescence-activated cell sorting (FACS) [53]/Dynal Invitrogen [54]/CTC-iChip [55]/Ephesia [56]/GEDI [57]/QMS [58]
 Positive selection Cell search [39]/Magnetic-activated cell sorting (MACS) [52]/Fluorescence-activated cell sorting (FACS) [53]/Epic system [59] Magsweeper [60]/Rosettesep/Easysep/Cytoquest/Adnatest [61]/GILUPI Nanodetector [62]/Liquid Biopsy (Cynvenio) [45]/Dynal Invitrogen
Physical
 Size Label-free/Spiral/Vortex/Microfiltration/Vycap/IsoFlux (Fluxion) [62]/Rare cell Devices Isolation by SizE of Tumor/Trophoblastic Cells (ISET) [63]/DEPArray [64]/(Silicon Biosystems)/ApoStream (ApoCell) [65]/Clear cell Parsortix [66]/Flexible micro spring array (FMSA) [67]/fiber-optic array scanning technology (FAST) [68]/Metacell [69]/Resettable Cell Trap/CellSieve/FaCTCheckr/ScreenCell/ClearCell FX [70]
 Gradient density OncoQuick (Grenier Bio-One) Ficoll-Paque [71]/Rosettesep/CyteSealer/AccuCyte [72]
 Di-electrophoretic (DEP) DEP-FFF/LFFF-DEP [73, 74]
 Functional analysis EPISOT/Vita-Assay (Vitatex) [75]/Epithelial ImmunoSPOT [38]/in vivo photoacoustic (PA) flow cytometry (PAFC) [62]