Table 1 Enrichment/isolation approaches of CTCs based on the inherent characteristics
Total approach | Methods/kits |
|---|---|
Biological (Immuno-affinity) | |
Negative selection | Rosettesep [50]/Easysep [51]/Magnetic-activated cell sorting (MACS) [52]/Fluorescence-activated cell sorting (FACS) [53]/Dynal Invitrogen [54]/CTC-iChip [55]/Ephesia [56]/GEDI [57]/QMS [58] |
Positive selection | Cell search [39]/Magnetic-activated cell sorting (MACS) [52]/Fluorescence-activated cell sorting (FACS) [53]/Epic system [59] Magsweeper [60]/Rosettesep/Easysep/Cytoquest/Adnatest [61]/GILUPI Nanodetector [62]/Liquid Biopsy (Cynvenio) [45]/Dynal Invitrogen |
Physical | |
Size | Label-free/Spiral/Vortex/Microfiltration/Vycap/IsoFlux (Fluxion) [62]/Rare cell Devices Isolation by SizE of Tumor/Trophoblastic Cells (ISET) [63]/DEPArray [64]/(Silicon Biosystems)/ApoStream (ApoCell) [65]/Clear cell Parsortix [66]/Flexible micro spring array (FMSA) [67]/fiber-optic array scanning technology (FAST) [68]/Metacell [69]/Resettable Cell Trap/CellSieve/FaCTCheckr/ScreenCell/ClearCell FX [70] |
Gradient density | OncoQuick (Grenier Bio-One) Ficoll-Paque [71]/Rosettesep/CyteSealer/AccuCyte [72] |
Di-electrophoretic (DEP) | |
Functional analysis | EPISOT/Vita-Assay (Vitatex) [75]/Epithelial ImmunoSPOT [38]/in vivo photoacoustic (PA) flow cytometry (PAFC) [62] |