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Fig. 1 | Cancer Cell International

Fig. 1

From: CircRNA CDR1as/miR-641/HOXA9 pathway regulated stemness contributes to cisplatin resistance in non-small cell lung cancer (NSCLC)

Fig. 1

The expression patterns of circRNA CDR1as, miR-641 and HOXA9 in human NSCLC cell lines (A549, H1299 and Calu6) and their paired descendent cisplatin-resistant sub-lines (A549/DDP, H1299/DDP and Calu6/DDP). The above cells were cultured in standard conditions and subsequently stimulated with high-dose cisplatin for 48 h, cell proliferation was measured by a CCK-8 assay and b trypan blue staining method was employed to detect cell viability. The results indicated that A549/DDP, H1299/DDP and Calu6/DDP were more resistant to high-dose cisplatin treatment compared to the parental DDP-sensitive NSCLC cells. The expression levels of c circRNA CDR1as and e HOXA9 mRNA were upregulated, while d miR-641 was downregulated in DDP-resistant NSCLC cells compared to DDP-sensitive cells, determined by using Real-Time qPCR. f, g Western Blot results showed that HOXA9 protein levels were increased in DDP-resistant NSCLC cells. Each experiment repeated at least 3 times. **P < 0.01

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