Fig. 3

MRTF-A promoted the transactivity of the miR-155 by CArG box (a) SRF knockdown with siSRF downregulated SRF mRNA levels in SGC-7901 and MGC-803 cells. b SRF protein levels were decreased in SRF depleted SGC-7901 and MGC-803 cells. c SRF knockdown with siSRF downregulated miR-155 RNA levels in SGC-7901 and MGC-803 cells. d A scheme for the structure of the miR-155 promoter. The position of the CArG-box and the ChIP-qPCR fragment are indicated. e MRTF-A was enriched in the CArG-containing promoter of miR-155. ChIP was performed with MRTF-A antibodies; normal rabbit IgG were used as the negative control. ChIP assay products were analyzed by RT-qPCR. f Histone acetylation at the miR-155 promoter was increased following MRTF-A overexpression. ChIP was performed with rabbit polyclonal antibodies against acetylated histone H3K9. g Overexpression of MRTF-A promoted the recruitment of RNA polymerase II to the miR-155 promoter. Data are shown as the mean ± S.E.M, based on three to six independent experiments. **, p < 0.01