Fig. 2

RNF181 promotes cell proliferation and migration in triple negative breast cancer cells. a, b Depletion of RNF181 inhibits the proliferation of triple negative breast cancer cells. BT549 and MDA-MA-231 were transfected with siControl or siRNF181. There were two different siRNA be used. After 24 h, the assay was CCK8 used to determine the cellar metabolic activity at indicated time points after infection. Experiments were done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for cell growth comparison. c–f Wound-healing assay of BT549 and MDA-MB-231 cells were transfected with siControl or siRNF181. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **, P < 0.01, ***, P < 0.001. g, h RNF181 depletion inhibits TNBC cell migration in BT549 and MDA-MB-231 cells. Two independent siRNA were used in the study. Transwell was used to check the migration capacity. The cell number was counted and data are presented as ± SD. **, P < 0.01, ***, P < 0.001