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Fig. 6 | Cancer Cell International

Fig. 6

From: RNF181 modulates Hippo signaling and triple negative breast cancer progression

Fig. 6

The hypothesis model of RNF181 regulating Hippo signal in triple negative breast cancer. a In the presence of the proteasome inhibitor MG132, the degradation effect of RNF181 on YAP did not further decrease YAP protein levels. BT549 cells were transfected with siRNF181 or siControl. After 24 h, cells were treated with 10 µM MG132/vehicle for 6 h. Cell lysates were prepared for Western blot analysis. The results are representative for three independent experiments. b RNF181 decreased K48-linked poly-ubiquitination of YAP. HEK293T cells were transfected with 0.5 µg Flag-RNF181 or Flag vector, together with 1 µg HA-K48 Ubi and Myc-YAP plasmid. The cell extracts were immunoprecipitated with HA antibody. The K48 specific poly-ubiquitinated YAP was detected via western blotting analysis. c RNF181 protein is related to YAP, which promotes YAP target gene transcription by promoting YAP stability and inhibits YAP degradation by inducing YAP K48 linked polyubiquitination

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