Fig. 2

Impacts of circ_0005909 suppression on OS cell growth in vitro and in vivo. a QRT-PCR was executed to assess circ_0005909 and XPR1 mRNA expression in HOS and U2OS cells transfected with sh-circ#1, sh-circ#2, or sh-NC. b–f Effects of circ_0005909 downregulation on cell viability (b), colony formation (c), migration (d), invasion (e), and EMT (f) of HOS and U2OS cells were determined through CCK-8 assay, cell formation assay, transwell assay, or western blot analysis. g Tumor volume of the mice in the sh-circ#1 and sh-NC groups was measured once a week from day 7. h Tumor weight of the mice in the sh-circ#1 and sh-NC groups were assessed on day 35 after injection. *P < 0.05 and **P < 0.01