Fig. 2

LINC00441 acts as a sponge of miR-450b-5p in CC cells. a LINC00441 location in CC cells was determined by isolation of nucleus-cytoplasm fraction. b The expression of miRNA candidates in CC cells was assessed by qRT-PCR. c MiR-450b-5p expression was measured by qRT-PCR after knockdown of LINC00441. d MiR-450b-5p overexpression efficiency was tested by qRT-PCR. e–g Cell proliferation, apoptosis, migration and invasion were detected through colony formation, flow cytometry and transwell assays after overexpressing miR-450b-5p. h RIP assay to the enrichment of the indicated molecules in anti-Ago2 or anti-IgG group. i The binding site between miR-450b-5p and LINC00441 was predicted by starBase (left); luciferase reporter assay examined the luciferase activity of pmirGLO-LINC00441-Wt/Mut in miR-450b-5p mimics transfected cells (right). ^**P < 0.01