Fig. 3

RAB10 is the target gene of miR-450b-5p. a MRNA candidates were selected by starBase. b RAB10 knockdown efficiency was examined by qRT-PCR. c–e Cell viability, proliferation and apoptosis were tested by CCK-8, colony formation assay and flow cytometry assay in response to RAB10 silencing. f Transwell assays detected cell migration and invasion when downregulating RAB10. g RIP assay measured enrichment of the indicated molecules in anti-Ago2 or anti-IgG group. h RNA pull down assay examined the PCR products in Bio-miR-450b-5p no probe or Bio-miR-450b-5p probe group. i StarBase predicted the binding site of RAB10 and miR-450b-5p (left). Luciferase reporter assay examined luciferase activity of pmirGLO-RAB10-Wt/Mut in miR-450b-5p mimics transfected cells (right). ^**P < 0.01