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Fig. 1 | Cancer Cell International

Fig. 1

From: Gamma synuclein is a novel nicotine responsive protein in oral cancer malignancy

Fig. 1

Nicotine induced SNCG expression and EMT change in OSCC cells. a Time- and dose-dependent increase SNCG protein expression in nicotine treated YD8 and OEC-M1 cells. Nicotine treatment also up-regulated SNCG mRNA expression in YD8 and OEC-M1 cells detected by b RT-PCR and c Q-PCR. d The morphology of control and nicotine-treated YD8 and OEC-M1 cells was investigated after 24, 48, and 72 h under microscope. Nicotine exposed YD8 and OEC-M1 cells displayed spindle morphology. e Western blot analysis of mesenchymal markers expression, such as fibronectin and vimentin was significant increase in YD8 cells and OEC-M1 cells with the time-dependent manner, whereas expression of the epithelial markers, ZO-1 and E-cadherin was decreased in both nicotine-treated YD8 and OEC-M1 cells. Data represent mean ± SD of 3 independent experiments. Statistical analysis was performed with ANOVA. *P < 0.05, **P < 0.01

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