Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Knockdown of DEPDC1B inhibits the development of glioblastoma

Fig. 3

Comparison of proliferation, apoptosis and migration of GBM cells in ShDEPDC1B-2 and shCtrl groups. a Transfection efficiency for U87 and U251 cells was evaluated by expression of green fluorescent protein 72 h post-infection. b, c The specificity and validity of the lentivirus-mediated shRNA knockdown of DEPDC1B expression was verified by qRT-PCR (b) and western blot analysis (c). d Cell proliferation of U87 and U251 cells with or without knockdown of DEPDC1B was evaluated by MTT assay. e Flow cytometry analysis based on Annexin V-APC staining was utilized to detect the percentage of early apoptotic cell for U87 and U251 cells. The X axis indicated the cell apoptosis while the Y axis indicated the green fluorescence detected from the GFP tagged on lentivirus (shDEPDC1B-2 and shCtrl). f Cell migration of U87 and U251 cells with or without knockdown of DEPDC1B was evaluated by Transwell assay. The data were expressed as mean ± SD (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page